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Characteristics
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LIGAND DENSITY: approximately 2 mg Protein G /mL of drained gel
DYNAMIC BINDING CAPACITY: approximately 18 mg human IgG/ml of drained gel (calculated as the amount of human IgG adsorbed to the gel before flow exceeded 1% of the absorbance of the ingoing solution at 30 cm/hr and a 0.92 mg/ml sample concentration.)
BEAD STRUCTURE: 4% highly crossed linked agarose
BEAD SIZE RANGE: 45-185 μm
MAXIMUM LINEAR FLOW RATE: >1300 cm/hr at 0.05 MPa (0.5 bar) in an XK 16/20 column, bed height 5 cm
MAXIMUM OPERATING BACK PRESSURE: 0.1 MPa (1 bar, 14 psi)
pH WORKING RANGE: 2-9 (Note: Protein G may hydrolyze at low pH)
pH STABILITY: long term storage and cleaning in place- pH of 3-9
CHEMICAL STABILITY: IgG binding capacity and recovery maintained after store for 7 days at
1) 37oC in 1M acetic acid (pH 2.0), 2mM sodium phosphate, 1% sDS (pH 7.0), 6 M guanidine- HCL, 70% ethanol or
2) 2 hrs at room temperature in 0.1 M HCL, pH 1.0, 8 M urea, pH 10.5, 01 M Glycine-NaOH, ph 11
PHYSICAL STABILITY: Negligible volume variation due to changes in pH or ionic strength
SANITIZATION: Sanitize column using 70% ethanol
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