產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：ProteinA/GMagneticBeads

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  • 產(chǎn)品廠(chǎng)商：Biovision
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ProteinA/GMagneticBeads
詳情介紹：
Purpose Easy to use, high-binding capacity, non-adherent beads. Useful for immunoprecipitation and enrichment of IgG antibodies.
Specificity High affinity for Fc region of IgG antibodies from a variety of species. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.
Characteristics Support Characteristics: Paramagnetic, spherical, 6 % crosslinked agarose
Ligand: Recombinant fusion Protein A/G
Particle Size: 75 – 150 μm
Binding Capacity Generally: >10 mg human IgG/ml wet beads
Working Temperature: Room temperature
Storage Solution: PBS w/0.02% NaN3
ProductDetails: Bead Ligand Protein A,Protein G
ProductDetails: Bead Matrix Magnetic Agarose beads
ProductDetails: Bead Size 112 μm
Comment

Protein A/G Magnetic Beads are prepared by covalently coupling Recombinant fusion Protein A/G (contains eight IgG binding domains, ABIN412505) to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 10 mg of human IgG per ml of wet gel.
Reagent Preparation

Binding buffer: 50 mM Tris, 150 mM NaCl, pH 7.5
Wash buffer: 50 mM Tris, 150 mM NaCl, pH 7.5 (or add 1% Octylglucoside to this buffer) (Could also try 1X PBS as both binding and wash buffer)
Elution buffer: 0.1 M -0.2 M Glycine pH 2.5-3.1 (or 0.1 M citric acid, pH 2.5-3.1 or 2.5 % Acetic Acid)
Assay Procedure

Prepare the antibody solution by diluting the required amount of antibody in binding buffer before running the protocol.
1. Magnetic Bead Preparation (perform three times)
a. Dispense the required amount of magnetic beads into a 1.5 ml microfuge tube.
b. Place the tube in the magnetic rack and remove the storage solution.
c. Add 500 μl binding buffer.
d. Resuspend the beads.
e. Remove the liquid
2. Antibody Capture
a. Immediately add the antibody solution.
b. Resuspend and mix (slow end-over-end) for at least 15 minutes.
c. Remove the liquid.
3. Washing
a. Add 500 μl Binding Buffer containing 0.5 M NaCl; Remove the liquid.
b. Add 500 μl Binding Buffer; Remove the liquid.
4. Target Binding
a. Add sample diluted in binding buffer.
b. Incubate with slow end-over-end mixing for up to 60 minutes.
c. Remove and collect unbound fraction.
5. Washing ( perform three times)
a. Add 500 μl wash buffer
b. Remove liquid (save washes to troubleshoot)
6. Elution (perform three times)
a. Add 2 volumes elution buffer (vs. bead volume).
b. Completely resuspend beads and incubate at least 2 minutes.
c. Remove and collect elution fraction.
Restrictions For Research Use only
Format Liquid
Buffer Supplied as a 50% slurry in PBS with 0.02% sodium azide.
Storage 4 °C
Expiry Date 12 months