產(chǎn)品詳情

  • 產(chǎn)品名稱：ActiveHumanCaspasesSetII

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：Biovision
  • 產(chǎn)品文檔：
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簡單介紹：
ActiveHumanCaspasesSetII
詳情介紹：
Unit Definition One unit of the recombinant human caspase-1, -2, -9, and -10 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA, VDVAD-pNA, LEHD-pNA, and IETD-pNA, respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Sub Type Cocktail
Background Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes. Most of the pro-caspases are matured by proteolysis to yield large and small subunits. Active caspases are heterotetramers, each of which consists of two large and two small subunits.
Synonyms: Active Human Caspases Set, active human caspase, active caspases, human caspases,caspase enzyme, caspase protein,
Application Notes Recombinant human caspases were expressed in E. coli and purified. Active recombinant caspases are useful for screen of caspase inhibitors, study enzyme kinetics and regulation, determine target substrate specificity, and as a positive control in caspase activity assays. It's recommended to 1 unit per assay for analyzing caspase activity. For a complete caspase activity assay protocol, please refer to Caspase Activity Assay Kits.
Comment

physical form description: Lyophilized powder
Contains active caspase-1/-2/-9/-10.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstituted to 0.1-1 unit per μl in PBS.
Buffer 50 mM Hepes,?pH 7.2, 50 mM NaCl, 0.1?% Chaps, 10 mM EDTA, 5?% Glycerol, and 10 mM DTT.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months