產(chǎn)品詳情

  • 產(chǎn)品名稱：ActiveHumanCaspasesGroupI,ActiveHuman

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  • 產(chǎn)品廠商：Biovision
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ActiveHumanCaspasesGroupI,ActiveHuman
詳情介紹：
Unit Definition One unit of the active recombinant caspase-1,-4,-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5), respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Sub Type Cocktail
Background The caspase family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: Group I (contains caspase-1,-4,-5), Group II (contains caspase-2,-3,-7), and Group III (contains caspase-6,-8,-9,-10). Evidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. The group I caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5).
Synonyms: Active Human Caspases group, active human caspase, active caspases, human caspases, caspase enzyme, caspase protein,
Application Notes Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, and as positive controls in caspase activity assays and Western blot analysis. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase activity assay protocol, please refer to Fluorometric or Colorimetric Caspase Assay Kits.
Comment

physical form description: Lyophilized powder
Group I contains active caspase-1/-4/-5.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 unit/μl in PBS containing 15?% glycerol.
Buffer 50 mM Hepes,?pH 7.2, 50 mM NaCl, 0.1?% Chaps, 10 mM EDTA, 5?% Glycerol, and 10 mM DTT.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months