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  • 產品名稱：ProteinLAgaroseResin

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ProteinLAgaroseResin
詳情介紹：
Purpose Protein L Agarose is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins.
Specificity Protein L only binds to Ig containing kappa light chains type kappa I, III, and IV in human, kappa I in mouse and specific for certain kappa subgroups in other species. Protein L binds weakly to rabbit Ig and does not bind Ig from bovine, goat, sheep or Ig with lambda light chains. Protein L is not appropriate for general polyclonal antibody purification from serum, which contains a mixture of immunoglobulins containing different types of light chains.
Characteristics Ligand Recombinant protein L
Binding capacity 10-15 mg human IgG/ml
Matrix Spherical, 4% agarose
Average particle size 90μm(45-165μm)
Maximum linear flow >150 cm/h
Maximum Pressure 0.3 MPa
pH stability pH 3-9
ProductDetails: Bead Ligand Protein L
ProductDetails: Bead Matrix Agarose beads
ProductDetails: Bead Size 90 μm
Background Protein L is first isolated from the surface of bacterial species Peptostreptoccus magnus and now recombinant Protein L is produced. Unlike Protein A and Protein G, which bind primarily through Fc regions of immunoglobulins Protein L binds immunoglobulin kappa light chain and binds a wider range of Ig classes and subclasses. Since no part of the heavy chain is involved in the binding interaction, Protein L can bind to all classes of Ig (i.e., IgG, IgM, IgA, IgE, and IgD) and also the single chain variable fragments (Scfv) and Fab fragments.
Research Area Bacteria, Gram positive bacteria
Comment

Protein L Agarose is Protein L immobilized to 4% high crosslinked beaded agarose.
Restrictions For Research Use only
Format Liquid
Handling Advice Do not freeze the resin.
Storage 4 °C
Storage Comment For storage, keep the medium at 2°C to 8°C in a suitable bacteriostat, e.g. 20% ethanol and/or 0.02% sodium azide.