產(chǎn)品詳情
簡單介紹:
1KbDNALadder
詳情介紹:
Purpose | 1Kb DNA ladder (Quantitative) is ideal for determining the size of double-stranded DNA from 500 to 1000 base pairs. |
Detection Method | Fluorometric |
Characteristics |
The ladder consists of 10 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 2000bp and 5000bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 μL loading, all fragments except 2000bp and 5000bp are at 40ng. The 2000bp and 5000bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use. 10 DNA Fragments (bp): 500, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 8000, and 10000 2000bp and 5000 Bands: 100ng/5 μL Other Bands: 40ng/5 μL Range: 500bp-10kb Bands: 10 |
Application Notes |
Recommended Loading: 5 μL/Lane. Add 5 μL ladder directly to each well. You may need more or less than 5 μL of ladder, depending on well size and level of intensity needed to visualize the bands. Recommended Loading: 5 μL/Lane, 8cm, 1% Agarose Gel, 1×TAE, 7V/cm, 45min. |
Comment |
Do not heat before loading. |
Restrictions | For Research Use only |
Format | Liquid |
Storage | -20 °C |
Storage Comment | 1 year at room temperature, for long term storage, please keep at -20°C. |
Expiry Date | 12 months |
Supplier Images |
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