產(chǎn)品詳情
簡單介紹:
HumanIgMisotypecontrol(FITC)
詳情介紹:
Isotype | IgM |
Fragment | F(ab')2 fragement |
Characteristics |
Human IgM protein (Fab mu) (FITC) conjugate Source: Human serum Alternative Names: Immunoglobulin M protein (Human) (Fab mu) (FITC) |
Purification | Human IgM protein (Fab mu) (FITC) was purified by delipidation, salt fractionation, ion exchange chromatography and dialysis. |
Background | Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. It is the primary antibody against A and B antigens on red blood cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope - the antigen- - at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. |
Research Area | Secondary Antibodies, Immunology |
Application Notes | Optimal working dilutions should be determined experimentally by the investigator. |
Comment |
Excitation/Emission wavelength: 494 nm/514 nm |
Restrictions | For Research Use only |
Format | Lyophilized |
Concentration | Lot specific |
Buffer | Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA and 0.01 % NaN3. Immunoglobulin and protease free. |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice |
Product is photosensitive and should be protected from light. Avoid repeated freeze/thaw cycles. |
Storage | RT/-20 °C |
Storage Comment | Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage. |