產(chǎn)品詳情
簡(jiǎn)單介紹:
MouseImmunoglobulinG2a(IgG2a)isotypecontrol(FITC)
詳情介紹:
Isotype | IgG2a |
Specificity | DS-MB-03584 s suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. This antibody may not be suitable for intracellular staining on some cell types. DS-MB-03584 recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that DS-MB-03584 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues. |
Characteristics |
Mouse IgG2a Negative Control Immunogen: Activated rat T-helper cells |
Alternative Name | IgG2a |
Research Area | Secondary Antibodies |
Application Notes | Immunoassay (ELISA, Western blotting): 1: 5,000-1: 10,000 |
Comment |
Excitation/Emission wavelength: 494 nm/514 nm |
Restrictions | For Research Use only |
Concentration | 0.1 mg/mL |
Buffer | Phosphate buffered saline pH 7.2 |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice | Product is photosensitive and should be protected from light. |
Storage | 4 °C |