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產(chǎn)品名稱：HumanGastricCancerBiomarkerArrayGS1
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產(chǎn)品廠商：RayBiotech
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HumanGastricCancerBiomarkerArrayGS1

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Product details
Product details
Purpose	G-Series Human Gastric Cancer Biomarker Array 1 Kit. Semi-quantitative measurement of 5 human gastric cancer biomarkers. Suitable for all liquid sample types.
Sample Type	Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell Lysate, Tissue Lysate
Analytical Method	Semi-Quantitative
Detection Method	Fluorometric
Specificity	CA19-9, CA72-4, CEA, Pepsinogen 1, Pepsinogen 2
Characteristics	High sensitivity (pg/mL to ng/mL levels) High specificity and system reproducibility Suitable for diverse sample types Low sample volume requirement: 50 μL or less Get results same day (6-hour processing time) Well-suited for high throughput assays Test 16 samples on each slide Data analysis software provides one-step computation
Components	Cytokine Antibody Array glass slide (4 or 8 arrays per slide) Biotinylated Detection Antibodies Streptavidin-conjugated HiLytePlus? 555 Fluor Blocking Buffer 20X Wash Buffer I 20X Wash Buffer II 2X Cell Lysis Buffer G-Series Antibody Array accessories Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film
Material not included	Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner

Application Details
Application Details
Application Notes	Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70?μl 6="" 7="" 10="" 13="" of="" sample="" or="" reagent="" is="" used.="" several="" incubation="" steps="" such="" as="" step="" (blocking),="" (sample="" incubation),="" (detection="" antibody="" (cy3="" equivalent="" dyestreptavidin="" incubation)="" may="" be="" done="" overnight="" at="" 4?°c.="" please="" make="" sure="" to="" cover="" the="" chamber="" tightly="" prevent="" evaporation.="" <="" td="">
Comment	The G-Series arrays feature fluorescent signal detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection. All G-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.
Sample Volume	100 μL
Plate	Glass Slide
Protocol	Dry the glass slide Block array surface Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Restrictions	For Research Use only

Handling
Handling
Handling Advice	Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The G-Series slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with a fine point permanent marker. Please write the number on the very bottom edge of the slide, taking care to avoid writing on the array well areas.
Storage	-20 °C
Storage Comment	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.