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Isotype & Other Controls(同型對照,其他對照)
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產(chǎn)品名稱:HumanIgGIsotypeControl
產(chǎn)品型號:
產(chǎn)品廠商:BioLeaf
產(chǎn)品文檔:
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簡單介紹:
HumanIgGIsotypeControl
詳情介紹:
Product details
Product details
Isotype
IgG
Fragment
Whole molecule
Characteristics
Purified Human IgG (Whole Molecule) Control is purified from normal human serum through Protein G chromatography. Purified Human IgG (Whole Molecule) Control can be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Western and Dot blots), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reactions.
Purification
Protein G chromatography
Target details
Target details
Research Area
Immunology, Secondary Antibodies
Application Details
Application Details
Application Notes
Each Investigator should determine their own optimal working dilution for specific applications.
Restrictions
For Research Use only
Handling
Handling
Format
Lyophilized
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 4 mg/mL.
Concentration
4 mg/mL
Buffer
PBS, pH 7.4, containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid repeated freezing and thawing cycles.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
References
References
Product cited in:
van Zeijl, van Koppen, Surovtseva, de Gooyer, Plate, Conti, Karstens, Timmers, Saeed, Wiersinga, Miltenburg, Fliers, Boelen: "Complete inhibition of rhTSH-, Graves' disease IgG-, and M22-induced cAMP production in differentiated orbital fibroblasts by a low-molecular-weight TSHR antagonist."
in:
The Journal of clinical endocrinology and metabolism
, Vol. 97, Issue 5, pp. E781-5, 2012 (PubMed).
Calvert, Kalantarov, Chang, Trakht, Blair, Roehrig: "Human monoclonal antibodies to West Nile virus identify epitopes on the prM protein."
in:
Virology
, Vol. 410, Issue 1, pp. 30-7, 2011 (PubMed).