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    產(chǎn)品詳情

    • 產(chǎn)品名稱：HumanMANF/ARMETHumanCellsTransfectedLysate(positivecontrol)(denatured)

    • 產(chǎn)品型號：
    • 產(chǎn)品廠商：SinoBiological
    • 產(chǎn)品文檔：
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    簡單介紹：
    HumanMANF/ARMETHumanCellsTransfectedLysate(positivecontrol)(denatured)
    詳情介紹：
    Characteristics denatured
    Human Cells transfected lysate in which Human MANF / ARMET has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.

    Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation.
    Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

    The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5 % glycerol, 1 % sodium dodecylsulfate, 0.01 % bromophenol blue) containing 5 % b-mercaptoethanol, and lyophilized.
    Sterility Non-sterile
    Alternative Name MANF
    Application Notes WB: Use at an assay dependent dilution.
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    Protocol 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
    2. Re-dissolve the pellet using 200 μL pure water and boiled for 2-5 min.
    3. Store it at -80 °C.
    Restrictions For Research Use only
    Format Lyophilized
    Reconstitution Reconstitute in pure water
    Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl?pH 7.4, 150 mM NaCl, 1?mM EDTA, 1?% Triton X-100, 0.1?% SDS, 1?% Sodium deoxycholate, 1?mM PMSF.
    Handling Advice Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
    Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    Storage 4 °C/-80 °C
    Storage Comment Store at 4°C. After re-dissolution, aliquot and store at -80°C.
    Expiry Date 12 months